Anterior cruciate ligament-derived mesenchymal stromal cells have a propensity to differentiate into the ligament lineage.

ABSTRACT:

Introduction

The anterior cruciate ligament (ACL) consists of various components, such as collagen, elastin fibres, and fibroblasts. Because ACL has a poor regenerative ability, ACL reconstruction need require the use of autologous tendons. In recent years, tissue-resident stem cells have been studied to promote ACL regeneration as an alternatively method. However, the existence of stem cells in ligaments has not been clearly defined. Here, we prospectively isolated stem cells from ACLs and characterized their properties.

Methods

ACLs from 11 donors and bone marrows (BM) from 8 donors were obtained with total knee arthroplasty. We used flow cytometry to screen the cell surface markers on ACL cells. Frozen sections were prepared from patient ACL tissues and stained with speci

SUBMITTER: Ogata Y 

PROVIDER: S-EPMC6149186 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature