Evaluation of GFP reporter utility for analysis of transcriptional slippage during gene expression.

ABSTRACT:

Background

Epimutations arising from transcriptional slippage seem to have more important role in regulating gene expression than earlier though. Since the level and the fidelity of transcription primarily determine the overall efficiency of gene expression, all factors contributing to their decrease should be identified and optimized.

Results

To examine the influence of A/T homopolymeric sequences on introduction of erroneous nucleotides by slippage mechanism green fluorescence protein (GFP) reporter was chosen. The in- or out-of-frame gfp gene was fused to upstream fragment with variable number of adenine or thymine stretches resulting in several hybrid GFP proteins with diverse amino acids at N-terminus. Here, by using T7 phage expression system we showed that the intensi

SUBMITTER: Wons E 

PROVIDER: S-EPMC6149199 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature