Anti-HIV Activity of Ocimum labiatum Extract and Isolated Pheophytin-a.

ABSTRACT: Ocimum plants are traditionally used to manage HIV/AIDS in various African countries. The effects of Ocimum labiatum extract on HIV-1 protease (PR) and reverse transcriptase (RT) is presented here along with characterization of an identified bioactive compound, achieved through ¹H- and ¹³C-NMR. The extract's effect on HIV-1 replication was assessed by HIV-1 p24 antigen capture. Cytotoxicity of samples was evaluated using tetrazolium dyes and real-time cell electronic sensing (RT-CES). Ocimum labiatum inhibited HIV-1 PR with an IC₅₀ value of 49.8 ± 0.4 μg/mL and presented weak inhibition (21%) against HIV-1 RT. The extract also reduced HIV-1 replication in U1 cells at a non-cytotoxic concentration (25 μg/mL). The CC₅₀ value of the extrac

SUBMITTER: Kapewangolo P 

PROVIDER: S-EPMC6150305 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature