Nanoplasmonic Sensing and Capillary Electrophoresis for Fast Screening of Interactions between Phosphatidylcholine Biomembranes and Surfactants.

ABSTRACT: Nanoplasmonic sensing (NPS), based on localized surface plasmon resonance, with sensors composed of glass covered with golden nanodisks and overlaid with a SiO₂ coating was applied in this study. Egg phosphatidylcholine (eggPC), being an easily accessible membrane-forming lipid, was used for preparation of biomimicking membranes. Small unilamellar vesicles with an approximate hydrodynamic diameter of 30 nm, formed by sonication in 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid buffer, were adsorbed within 10 min on the sensor surface either as intact vesicles or as a planar bilayer. The adsorbed biomembrane systems were further utilized for interaction studies with four different well-known surfactants (negatively and positively charged, zwitterionic, and nonionic) and each

SUBMITTER: Dusa F 

PROVIDER: S-EPMC6150717 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature