Image velocimetry and spectral analysis enable quantitative characterization of larval zebrafish gut motility.

ABSTRACT:

Background

Normal gut function requires rhythmic and coordinated movements that are affected by developmental processes, physical and chemical stimuli, and many debilitating diseases. The imaging and characterization of gut motility, especially regarding periodic, propagative contractions driving material transport, are therefore critical goals. Previous image analysis approaches have successfully extracted properties related to the temporal frequency of motility modes, but robust measures of contraction magnitude, especially from in vivo image data, remain challenging to obtain.

Methods

We developed a new image analysis method based on image velocimetry and spectral analysis that reveals temporal characteristics such as frequency and wave propagation speed, while also provi

SUBMITTER: Ganz J 

PROVIDER: S-EPMC6150784 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature