A microwell culture system that allows group culture and is compatible with human single media.

ABSTRACT:

Purpose

A microwell culture system that facilitates group culture, such as well-of-the-well (WOW), improves embryonic development in an individual culture. We examined the effect of WOW on embryonic development in vitro with commercially available human single culture media.

Methods

Using four different commercial human single culture media, in vitro development and imprinted gene expression of bovine embryos cultured in WOW were compared to droplet culture (one zygote per drop). To determine the effects of microwell and group culture on embryonic development, different numbers of embryos were cultured in droplet or WOW. Diffusion simulation of accumulating metabolites was conducted using the finite volume method.

Results

WOW had a positive effect on bovine embryonic

SUBMITTER: Ieda S 

PROVIDER: S-EPMC6150891 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature