Chromosome Segregation Fidelity in Epithelia Requires Tissue Architecture.

ABSTRACT: Much of our understanding of chromosome segregation is based on cell culture systems. Here, we examine the importance of the tissue environment for chromosome segregation by comparing chromosome segregation fidelity across several primary cell types in native and nonnative contexts. We discover that epithelial cells have increased chromosome missegregation outside of their native tissues. Using organoid culture systems, we show that tissue architecture, specifically integrin function, is required for accurate chromosome segregation. We find that tissue architecture enhances the correction of merotelic microtubule-kinetochore attachments, and this is especially important for maintaining chromosome stability in the polyploid liver. We propose that disruption of tissue architecture could unde

SUBMITTER: Knouse KA 

PROVIDER: S-EPMC6151153 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature