Cellular Uptake and Delivery-Dependent Effects of Tb³⁺-Doped Hydroxyapatite Nanorods.

ABSTRACT: With the increasing interest in hydroxyapatite (HA) nanostructures for use in biomedicine, the systematic evaluation of their potential effects on biological systems is becoming critically important. In this work, we report the in vitro cellular uptake, in vivo tissue distributions and toxicity of Tb³⁺-doped HA (HA-Tb) after short-, intermediate-, and long-term exposure. Transmission electron microscopy analysis indicated that HA-Tb was taken up by cells via vesicle endocytosis. Cell proliferation and cytotoxicity assay, combined with confocal laser scanning microscopy, indicated excellent cell viability with no changes in cell morphology at the examined doses. Three HA-Tb delivery methods (intraperitoneal, intragastric, and intravenous) resulted in similar time-dependent tissue

SUBMITTER: Wei Y 

PROVIDER: S-EPMC6152145 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature