Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers.

ABSTRACT:

Background

Lung epithelial lining fluid (ELF)-sampled through sputum induction-is a medium rich in cells, proteins and lipids. However, despite its key role in maintaining lung function, homeostasis and defences, the composition and biology of ELF, especially in respect of lipids, remain incompletely understood.

Objectives

To characterise the induced sputum lipidome of healthy adult individuals, and to examine associations between different ELF lipid phenotypes and the demographic characteristics within the study cohort.

Methods

Induced sputum samples were obtained from 41 healthy non-smoking adults, and their lipid compositions analysed using a combination of untargeted shotgun and liquid chromatography mass spectrometry methods. Topological data analysis (TDA) was u

SUBMITTER: Brandsma J 

PROVIDER: S-EPMC6153688 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature