The influence of neuronal electrical activity on the mammalian central clock metabolome.

ABSTRACT:

Introduction

Most organisms display circadian rhythms in physiology and behaviour. In mammals, these rhythms are orchestrated by the suprachiasmatic nucleus (SCN). Recently, several metabolites have emerged as important regulators of circadian timekeeping. Metabolomics approaches have aided in identifying some key metabolites in circadian processes in peripheral tissue, but methods to routinely measure metabolites in small brain areas are currently lacking.

Objective

The aim of the study was to establish a reliable method for metabolite quantifications in the central circadian clock and relate them to different states of neuronal excitability.

Methods

We developed a method to collect and process small brain tissue samples (0.2 mm³), suitable for liquid chro

SUBMITTER: Buijink MR 

PROVIDER: S-EPMC6153692 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature