Effects of Phage Endolysin SAL200 Combined with Antibiotics on Staphylococcus aureus Infection.

ABSTRACT: Phages and their derivatives are increasingly being reconsidered for use in the treatment of bacterial infections due to the rising rates of antibiotic resistance. We assessed the antistaphylococcal effect of the endolysin SAL200 in combination with standard-of-care (SOC) antibiotics. The activity of SAL200 when it was combined with SOC antibiotics was assessed in vitro by checkerboard and time-kill assays and in vivo with murine bacteremia and Galleria mellonella infection models. SAL200 reduced the SOC antibiotic MICs and showed a ≥3-log₁₀-CFU/ml reduction of Staphylococcus aureus counts within 30 min in time-kill assays. Combinations of SAL200 and SOC antibiotics achieved a sustained decrease of >2 log₁₀ CFU/ml. SAL200 significantly lowe

SUBMITTER: Kim NH 

PROVIDER: S-EPMC6153796 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature