Analysis of Drosophila melanogaster testis transcriptome.

ABSTRACT:

Background

The formation of matured and individual sperm involves a series of molecular and spectacular morphological changes of the developing cysts in Drosophila melanogaster testis. Recent advances in RNA Sequencing (RNA-Seq) technology help us to understand the complexity of eukaryotic transcriptomes by dissecting different tissues and developmental stages of organisms. To gain a better understanding of cellular differentiation of spermatogenesis, we applied RNA-Seq to analyse the testis-specific transcriptome, including coding and non-coding genes.

Results

We isolated three different parts of the wild-type testis by dissecting and cutting the different regions: 1.) the apical region, which contains stem cells and developing spermatocytes 2.) the middle region, with enri

SUBMITTER: Vedelek V 

PROVIDER: S-EPMC6154878 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature