Identification of active and taxonomically diverse 1,4-dioxane degraders in a full-scale activated sludge system by high-sensitivity stable isotope probing.

ABSTRACT: 1,4-Dioxane is one of the most common and persistent artificial pollutants in petrochemical industrial wastewaters and chlorinated solvent groundwater plumes. Despite its possible biological treatment in natural environments, the identity and dynamics of the microorganisms involved are largely unknown. Here, we identified active and diverse 1,4-dioxane-degrading microorganisms from activated sludge by high-sensitivity stable isotope probing of rRNA. By rigorously analyzing 16S rRNA molecules in RNA density fractions of ¹³C-labeled and unlabeled 1,4-dioxane treatments, we discovered 10 significantly ¹³C-incorporating microbial species from the complex microbial community. 16S rRNA expression assays revealed that 9 of the 10 species, including the well-known degrader Ps

SUBMITTER: Aoyagi T 

PROVIDER: S-EPMC6155002 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature