The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen.

ABSTRACT: We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals.

SUBMITTER: Sander V 

PROVIDER: S-EPMC6155399 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature