Functional and Biochemical Characterization of Dib1's Role in Pre-Messenger RNA Splicing.

ABSTRACT: The spliceosome is a dynamic macromolecular machine that undergoes a series of conformational rearrangements as it transitions between the several states required for accurate splicing. The transition from the B to B^act is a key part of spliceosome assembly and is defined by the departure of several proteins, including essential U5 component Dib1. Recent structural studies suggest that Dib1 has a role in preventing premature spliceosome activation, as it is positioned adjacent to the U6 snRNA ACAGAGA and the U5 loop I, but its mechanism is unknown. Our data indicate that Dib1 is a robust protein that tolerates incorporation of many mutations, even at positions thought to be key for its folding stability. However, we have identified two temperature-sensitive mutants that stall in

SUBMITTER: Schreib CC 

PROVIDER: S-EPMC6155989 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature