Filter-Aided Sample Preparation Procedure for Mass Spectrometric Analysis of Plant Histones.

ABSTRACT: Characterization of histone post-translational modifications (PTMs) is still challenging, and robust histone sample preparation is essential for convincing evaluation of PTMs by mass spectrometry. An effective protocol for extracting plant histone proteins must also avoid excessive co-extraction of the numerous potential interfering compounds, including those related to secondary metabolism. Currently, the co-existence of histone marks is addressed mostly by shotgun proteomic analysis following chemical derivatization of histone lysine residues. Here, we report a straightforward approach for plant histone sample preparation for mass spectrometry, based on filter-aided sample preparation coupled with histone propionylation. The approach offers savings in sample handling and preparation time

SUBMITTER: Ledvinova D 

PROVIDER: S-EPMC6156276 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature