Development of an AmpliSeq^TM Panel for Next-Generation Sequencing of a Set of Genetic Predictors of Persisting Pain.

ABSTRACT: Background: Many gene variants modulate the individual perception of pain and possibly also its persistence. The limited selection of single functional variants is increasingly being replaced by analyses of the full coding and regulatory sequences of pain-relevant genes accessible by means of next generation sequencing (NGS). Methods: An NGS panel was created for a set of 77 human genes selected following different lines of evidence supporting their role in persisting pain. To address the role of these candidate genes, we established a sequencing assay based on a custom AmpliSeq^TM panel to assess the exomic sequences in 72 subjects of Caucasian ethnicity. To identify the systems biology of the genes, the biological functions associated with these genes were assesse

SUBMITTER: Kringel D 

PROVIDER: S-EPMC6156278 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature