Bradykinin-mediated Ca²⁺ signalling regulates cell growth and mobility in human cardiac c-Kit⁺ progenitor cells.

ABSTRACT: Our recent study showed that bradykinin increases cell cycling progression and migration of human cardiac c-Kit⁺ progenitor cells by activating pAkt and pERK1/2 signals. This study investigated whether bradykinin-mediated Ca²⁺ signalling participates in regulating cellular functions in cultured human cardiac c-Kit⁺ progenitor cells using laser scanning confocal microscopy and biochemical approaches. It was found that bradykinin increased cytosolic free Ca²⁺ ( Cai2+ ) by triggering a transient Ca²⁺ release from ER IP3Rs followed by sustained Ca²⁺ influx through store-operated Ca²⁺ entry (SOCE) channel. Blockade of B2 receptor with HOE140 or IP3Rs with araguspongin B or silencing IP3R3 with siRNA abolished both Ca

SUBMITTER: Li G 

PROVIDER: S-EPMC6156395 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature