Apoptotic efficacy of multifaceted biosynthesized silver nanoparticles on human adenocarcinoma cells.

ABSTRACT: Metallic nanoparticles (NPs) especially silver (Ag) NPs have shown immense potential in medical applications due to their distinctive physio-chemical and biological properties. This article reports the conjugation of Ag NPs with Rubus fairholmianus extract. The modification of Ag NPs was confirmed using various physico-chemical characterization techniques. The cytotoxic effect of Rubus-conjugated Ag NPs (RAgNPs) was studied by LDH assay and proliferation by ATP assay. The apoptotic inducing ability of the NPs were investigated by Annexin V/PI staining, caspase 3/7 analysis, cytochrome c release, intracellular ROS analysis, Hoechst staining and mitochondrial membrane potential analysis using flow cytometry. The expression of apoptotic proteins caspase 3, Bax and P53 were analyzed using ELIS

SUBMITTER: Plackal Adimuriyil George B 

PROVIDER: S-EPMC6156419 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature