Glutamate-activated BK channel complexes formed with NMDA receptors.

ABSTRACT: The large-conductance calcium- and voltage-activated K⁺ (BK) channel has a requirement of high intracellular free Ca²⁺ concentrations for its activation in neurons under physiological conditions. The Ca²⁺ sources for BK channel activation are not well understood. In this study, we showed by coimmunopurification and colocalization analyses that BK channels form complexes with NMDA receptors (NMDARs) in both rodent brains and a heterologous expression system. The BK-NMDAR complexes are broadly present in different brain regions. The complex formation occurs between the obligatory BKα and GluN1 subunits likely via a direct physical interaction of the former's intracellular S0-S1 loop with the latter's cytosolic regions. By patch-clamp recording on mouse brain

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6156621 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature