Recapitulation of hepatitis B virus-host interactions in liver organoids from human induced pluripotent stem cells.

ABSTRACT: Therapies against hepatitis B virus (HBV) have improved in recent decades; however, the development of individualized treatments has been limited by the lack of individualized infection models. In this study, we used human induced pluripotent stem cell (hiPSC) to generate a functional liver organoid (LO) that inherited the genetic background of the donor, and evaluated its application in modeling HBV infection and exploring virus-host interactions. To establish a functional hiPSC-LO, we cultured hiPSC-derived endodermal, mesenchymal, and endothelial cells with a chemically defined medium in a three-dimensional microwell culture system. Based on cell-cell interactions, these cells could organize themselves and gradually differentiate into a functional organoid, which exhibited stronger hepa

SUBMITTER: Nie YZ 

PROVIDER: S-EPMC6156717 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature