Identification of YTH Domain-Containing Proteins as the Readers for N1-Methyladenosine in RNA.

ABSTRACT: N1-methyladenosine (m¹A) is an important post-transcriptional modification in RNA; however, the exact biological role of m¹A remains to be determined. By employing a quantitative proteomics method, we identified multiple putative protein readers of m¹A in RNA, including several YTH domain family proteins. We showed that YTHDF1-3 and YTHDC1, but not YTHDC2, could bind directly to m¹A in RNA. We also found that Trp⁴³² in YTHDF2, a conserved residue in the hydrophobic pocket of the YTH domain that is necessary for its binding to N⁶-methyladenosine (m⁶A), is required for its recognition of m¹A. An analysis of previously published data revealed transcriptome-wide colocalization of YTH domain-containing prote

SUBMITTER: Dai X 

PROVIDER: S-EPMC6157021 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature