Serum and Ectopic Endometrium from Women with Endometriosis Modulate Macrophage M1/M2 Polarization via the Smad2/Smad3 Pathway.

ABSTRACT:

Objective

This study investigated the alterations in macrophage polarization in patients with endometriosis as well as the underlying molecular mechanisms.

Methods

Peritoneal washings, serum samples, and endometrial tissues were collected from endometriosis patients and control subjects. Endometrial stromal cells (ESCs) were isolated from endometrial tissue, and conditioned medium was prepared by treating ESCs with or without various concentrations of interleukin- (IL-) 6, estrogen, or progestin. The frequencies of CD86+ and CD163+ cells and expression levels of these markers as well as the cytokines IL-12 and IL-10 were measured in THP-1- (human monocytic leukemia cell) derived macrophages.

Results

There was a decrease in the percentage of CD86+ macrophages in the pe

SUBMITTER: Nie MF 

PROVIDER: S-EPMC6157144 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature