Relative Level of Bacteriophage Multiplication in vitro or in Phyllosphere May Not Predict in planta Efficacy for Controlling Bacterial Leaf Spot on Tomato Caused by Xanthomonas perforans.

ABSTRACT: Following analysis of eight phages under in vitro, growth chamber and greenhouse conditions with the bacterial spot of tomato pathogen Xanthomonas perforans, there was no correlation between disease control efficacy and in vitro phage multiplication, in vitro bacterial suppression, or in vivo phage multiplication in the presence of the host, but there was a low correlation between phage persistence on the leaf surface and disease control. Two of the 8 virulent phages (ΦXv3-21 and ΦXp06-02) were selected for in depth analysis with two X. perforans (Xp06-2-1 and Xp17-12) strains. In in vitro experiments, phage ΦXv3-21 was equally effective in infecting the two bacterial strains based on efficiency of plating (EOP). Phage ΦXp06-02, on the other

SUBMITTER: Balogh B 

PROVIDER: S-EPMC6157332 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature