Cucumber (Cucumis sativus L.) Seedling Rhizosphere Trichoderma and Fusarium spp. Communities Altered by Vanillic Acid.

ABSTRACT: Root exudates mediate soil microbiome composition and diversity, which might further influence plant development and health. Vanillic acid from root exudates is usually referred as autotoxin of cucumber, however, how vanillic acid affect soil microbial community diversities and abundances remains unclear. In this study, vanillic acid (VA; 0.02, 0.05, 0.1, and 0.2 μmol g^-1 soil) was applied to soil every other day for a total of five applications. We used Illumina MiSeq sequencing, quantitative PCR (qPCR) and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) to test the effects of VA on the total fungi community composition as well as the Trichoderma and Fusarium spp. community abundances and structures in the cucumber rhizosphere. Illumina MiSeq sequencing sho

SUBMITTER: Chen S 

PROVIDER: S-EPMC6157394 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature