An Experimentally Determined State Diagram for Human CD4⁺ T Lymphocyte CXCR4-Stimulated Adhesion Under Shear Flow.

ABSTRACT:

Introduction

The leukocyte adhesion cascade is important for the maintenance of homeostasis and the ability of immune cells to access sites of infection and inflammation. Despite much work identifying the molecular components of the cascade, and numerous simulations to predict the relationship between molecule density, identity, and adhesion, these relationships have not been measured experimentally.

Methods

Using surfaces functionalized with recombinant ICAM-1 and/or E-selectin along with immobilized SDF-1α, we used a flow chamber to measure rates of tethering, rolling and arrest of primary naïve human CD4⁺ T lymphocytes on different surface densities of ligand.

Results

Cells required a minimum level of ligand density to progress beyond tethering. E-select

SUBMITTER: Anderson NR 

PROVIDER: S-EPMC6157745 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature