Age-specific changes in genome-wide methylation enrich for Foxa2 and estrogen receptor alpha binding sites.

ABSTRACT: The role of DNA methylation patterns in complex phenotypes remains unclear. To explore this question, we adapted our methods for rare variant analysis to characterize genome-wide murine DNA hybridization array to investigate methylation at CpG islands, shores, and regulatory elements. We have applied this platform to compare age and tissue- specific methylation differences in the brain and spleen of young and aged mice. As expected from prior studies, there are clear global differences in organ-specific, but not age-specific, methylation due mostly to changes at repetitive elements. Surprisingly, out of 200,000 loci there were only 946 differentially methylated cytosines (DMCs) between young and old samples (529 hypermethylated, 417 hypomethylated in aged mice) compared to thousands of tis

SUBMITTER: Uli N 

PROVIDER: S-EPMC6157835 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature