Comparative proteomic analyses of human adipose extracellular matrices decellularized using alternative procedures.

ABSTRACT: Decellularized human adipose tissue has potential clinical utility as a processed biological scaffold for soft tissue cosmesis, grafting, and reconstruction. Adipose tissue decellularization has been accomplished using enzymatic-, detergent-, and/or solvent-based methods. To examine the hypothesis that distinct decellularization processes may yield scaffolds with differing compositions, the current study employed mass spectrometry to compare the proteomes of human adipose-derived matrices generated through three independent methods combining enzymatic-, detergent-, and/or solvent-based steps. In addition to protein content, bioscaffolds were evaluated for deoxyribose nucleic acid depletion, extracellular matrix composition, and physical structure using optical density, histochemical staini

SUBMITTER: Thomas-Porch C 

PROVIDER: S-EPMC6158104 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature