TGF-β receptor 1 regulates progenitors that promote browning of white fat.

ABSTRACT:

Objective

Beige/brite adipose tissue displays morphological characteristics and beneficial metabolic traits of brown adipose tissue. Previously, we showed that TGF-β signaling regulates the browning of white adipose tissue. Here, we inquired whether TGF-β signals regulated presumptive beige progenitors in white fat and investigated the TGF-β regulated mechanisms involved in beige adipogenesis.

Methods

We deleted TGF-β receptor 1 (TβRI) in adipose tissue (TβRI^AdKO mice) and, using flow-cytometry based assays, identified and isolated presumptive beige progenitors located in the stromal vascular cells of white fat. These cells were molecularly characterized to examine beige/brown marker expression and to investigate TGF-β dependent mechanisms. Further, the cells were

SUBMITTER: Wankhade UD 

PROVIDER: S-EPMC6158128 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature