Micropatterning of planar metal electrodes by vacuum filling microfluidic channel geometries.

ABSTRACT: We present a simple, facile method to micropattern planar metal electrodes defined by the geometry of a microfluidic channel network template. By introducing aqueous solutions of metal into reversibly adhered PDMS devices by desiccation instead of flow, we are able to produce difficult to pattern "dead end" or discontinuous features with ease. We characterize electrodes fabricated using this method and perform electrical lysis of mammalian cancer cells and demonstrate their use as part of an antibody capture assay for GFP. Cell lysis in microwell arrays is achieved using the electrodes and the protein released is detected using an antibody microarray. We show how the template channels used as part of the workflow for patterning the electrodes may be produced using photolithography-free met

SUBMITTER: Chatzimichail S 

PROVIDER: S-EPMC6158193 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature