Blocking mechanosensitive ion channels eliminates the effects of applied mechanical loading on chick joint morphogenesis.

ABSTRACT: Abnormalities in joint shape are increasingly considered a critical risk factor for developing osteoarthritis in life. It has been shown that mechanical forces during prenatal development, particularly those due to fetal movements, play a fundamental role in joint morphogenesis. However, how mechanical stimuli are sensed or transduced in developing joint tissues is unclear. Stretch-activated and voltage-gated calcium ion channels have been shown to be involved in the mechanoregulation of chondrocytes in vitro In this study, we analyse, for the first time, how blocking these ion channels influences the effects of mechanical loading on chick joint morphogenesis. Using in vitro culture of embryonic chick hindlimb explants in a mechanostimulation bioreactor, we block stretch-activated and volt

SUBMITTER: Parisi C 

PROVIDER: S-EPMC6158207 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature