Heterocyclic sterol probes for live monitoring of sterol trafficking and lysosomal storage disorders.

ABSTRACT: The monitoring of intracellular cholesterol homeostasis and trafficking is of great importance because their imbalance leads to many pathologies. Reliable tools for cholesterol detection are in demand. This study presents the design and synthesis of fluorescent probes for cholesterol recognition and demonstrates their selectivity by a variety of methods. The construction of dedicated library of 14 probes was based on heterocyclic (pyridine)-sterol derivatives with various attached fluorophores. The most promising probe, a P1-BODIPY conjugate FP-5, was analysed in detail and showed an intensive labelling of cellular membranes followed by intracellular redistribution into various cholesterol rich organelles and vesicles. FP-5 displayed a stronger signal, with faster kinetics, than the commer

SUBMITTER: Kralova J 

PROVIDER: S-EPMC6158244 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature