Peripheral re-localization of constitutive heterochromatin advances its replication timing and impairs maintenance of silencing marks.

ABSTRACT: The replication of the genome is a highly organized process, both spatially and temporally. Although a lot is known on the composition of the basic replication machinery, how its activity is regulated is mostly unknown. Several chromatin properties have been proposed as regulators, but a potential role of the nuclear DNA position remains unclear. We made use of the prominent structure and well-defined heterochromatic landscape of mouse pericentric chromosome domains as a well-studied example of late replicating constitutive heterochromatin. We established a method to manipulate its nuclear position and evaluated the effect on replication timing, DNA compaction and epigenetic composition. Using time-lapse microscopy, we observed that constitutive heterochromatin, known to replicate during l

SUBMITTER: Heinz KS 

PROVIDER: S-EPMC6158597 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature