Direct transfection of clonal organoids in Matrigel microbeads: a promising approach toward organoid-based genetic screens.

ABSTRACT: Organoid cultures in 3D matrices are relevant models to mimic the complex in vivo environment that supports cell physiological and pathological behaviors. For instance, 3D epithelial organoids recapitulate numerous features of glandular tissues including the development of fully differentiated acini that maintain apico-basal polarity with hollow lumen. Effective genetic engineering in organoids would bring new insights in organogenesis and carcinogenesis. However, direct 3D transfection on already formed organoids remains challenging. One limitation is that organoids are embedded in extracellular matrix and grow into compact structures that hinder transfection using traditional techniques. To address this issue, we developed an innovative approach for transgene expression in 3D organoids b

SUBMITTER: Laperrousaz B 

PROVIDER: S-EPMC6158603 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature