Engineering a Rugged Nanoscaffold To Enhance Plug-and-Display Vaccination.

ABSTRACT: Nanoscale organization is crucial to stimulating an immune response. Using self-assembling proteins as multimerization platforms provides a safe and immunogenic system to vaccinate against otherwise weakly immunogenic antigens. Such multimerization platforms are generally based on icosahedral viruses and have led to vaccines given to millions of people. It is unclear whether synthetic protein nanoassemblies would show similar potency. Here we take the computationally designed porous dodecahedral i301 60-mer and rationally engineer this particle, giving a mutated i301 (mi3) with improved particle uniformity and stability. To simplify the conjugation of this nanoparticle, we employ a SpyCatcher fusion of mi3, such that an antigen of interest linked to the SpyTag peptide can spontaneously cou

SUBMITTER: Bruun TUJ 

PROVIDER: S-EPMC6158681 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature