Genome analysis of methicillin resistance in Macrococcus caseolyticus from dairy cattle in England and Wales.

ABSTRACT: Species of the genus Macrococcus are widespread commensals of animals but are becoming increasingly recognised as veterinary pathogens. They can encode methicillin resistance and are implicated in its spread to the closely-related, but more pathogenic, staphylococci. In this study we have identified 33 isolates of methicillin-resistant Macrococcus caseolyticus from bovine bulk tank milk from England and Wales. These isolates were characterised to provide insight into the molecular epidemiology of M. caseolyticus and to discern the genetic basis for their methicillin resistance. Antimicrobial susceptibility testing was performed by Vitek2 and disc diffusion. Isolates were whole-genome sequenced to evaluate phylogenetic relationships and the presence of methicillin resistance determinants, m

SUBMITTER: MacFadyen AC 

PROVIDER: S-EPMC6159548 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature