MOB-suite: software tools for clustering, reconstruction and typing of plasmids from draft assemblies.

ABSTRACT: Large-scale bacterial population genetics studies are now routine due to cost-effective Illumina short-read sequencing. However, analysing plasmid content remains difficult due to incomplete assembly of plasmids. Bacterial isolates can contain any number of plasmids and assembly remains complicated due to the presence of repetitive elements. Numerous tools have been developed to analyse plasmids but the performance and functionality of the tools are variable. The MOB-suite was developed as a set of modular tools for reconstruction and typing of plasmids from draft assembly data to facilitate characterization of plasmids. Using a set of closed genomes with publicly available Illumina data, the MOB-suite identified contigs of plasmid origin with both high sensitivity and specificity (95 and

SUBMITTER: Robertson J 

PROVIDER: S-EPMC6159552 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature