Decreased expression of TRIM21 indicates unfavorable outcome and promotes cell growth in breast cancer.

ABSTRACT:

Background

Tripartite motif-containing protein 21 (TRIM21), an E3 ubiquitin ligase, has been implicated in autoimmune diseases. Dysregulation of TRIM21 contributes to the progression of human malignancies, but its role and clinical significance in breast cancer remain unclear.

Methods

The expression of TRIM21 was examined by quantitative real-time PCR, Western blot, and immunohistochemistry. The role of TRIM21 in the progression of breast cancer was determined using in vitro and in vivo models. The upstream regulation of TRIM21 was investigated by luciferase reporter assay.

Results

Here, we showed that TRIM21 expression in breast cancer tissues was decreased at both the mRNA and protein levels in comparison to that in nontumorous tissues. TRIM21 expression was closely

SUBMITTER: Zhou W 

PROVIDER: S-EPMC6159792 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature