Evaluation of the effect of ionic liquids as adjuvants in polymer-based aqueous biphasic systems using biomolecules as molecular probes.

ABSTRACT: Aqueous biphasic systems (ABS) have been largely investigated for the extraction, separation and/or purification of biomolecules. Recently, the use of ionic liquids (ILs) as additives in conventional polymer-based ABS was proposed to overcome the limited range of polarities of the coexisting phases. However, the impact of ILs on the partitioning of biomolecules on IL additivated ABS is not universal and is still poorly understood. Aiming at obtaining additional insights on this matter, the effects of the chemical structure of the IL, tie-line length (TLL) and biomolecule nature upon the partition of a series of model biomolecules were investigated. For this purpose, ternary ABS (composed of polyethylene glycol (PEG) 400, citrate buffer at pH 7.0, and water), and several quaternary ABS (com

SUBMITTER: de Cassia S Sousa R 

PROVIDER: S-EPMC6159875 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature