Immortalization of common marmoset monkey fibroblasts by piggyBac transposition of hTERT.

ABSTRACT: Following a certain type-specific number of mitotic divisions, terminally differentiated cells undergo proliferative senescence, thwarting efforts to expand different cell populations in vitro for the needs of scientific research or medical therapies. The primary cause of this phenomenon is the progressive shortening of the telomeres and the subsequent activation of cell cycle control pathways leading to a block of cell proliferation. Restoration of telomere length by transgenic expression of telomerase reverse transcriptase (TERT) usually results in bypassing of the replicative senescence and ultimately in cell immortalization. To date, there have not been any reports regarding immortalization of cells from common marmoset (Callithrix jacchus), an important non-human primate model for var

SUBMITTER: Petkov S 

PROVIDER: S-EPMC6160115 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature