A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination.

ABSTRACT: Site-specific recombinases (SSR) are utilized as important genome engineering tools to precisely modify the genome of mice and other model organisms. Reporter mice that mark cells that at any given time had expressed the enzyme are frequently used for lineage tracing and to characterize newly generated mice expressing a recombinase from a chosen promoter. With increasing sophistication of genome alteration strategies, the demand for novel SSR systems that efficiently and specifically recombine their targets is rising and several SSR-systems are now used in combination to address complex biological questions in vivo. Generation of reporter mice for each one of these recombinases is cumbersome and increases the number of mouse lines that need to be maintained in animal facilities. Here we pr

SUBMITTER: Karimova M 

PROVIDER: S-EPMC6160450 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature