Nearly complete genome sequence of one GII.17 Norovirus identified by direct sequencing from HuZhou, China.

ABSTRACT:

Background

Human norovirus is the leading cause of acute gastroenteritis worldwide. However, in vitro culture system is complicated for human norovirus. Sequence analysis became more useful for norovirus research, particularly when using complete genomic sequences.

Methods

Real-time RT-PCR (qPCR) was performed for norovirus detection. Three modified paris of PCR primes were designed based on the alignment of the novel GII.17 norovirus complete sequence available in Genbank., which could amplify three overlapping fragments cover the whole genome. The PCR fragments were sequencing by Sanger sequence with Primer walking methods. Genogroup and genotype were assigned using the Norovirus Noronet typing tool and the strains were named according to the time of isolation. The phyloge

SUBMITTER: Ji L 

PROVIDER: S-EPMC6160709 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature