Evaluation of antibody fragment properties for near-infrared fluorescence imaging of HER3-positive cancer xenografts.

ABSTRACT: In vivo imaging is influenced by the half-life, tissue penetration, biodistribution, and affinity of the imaging probe. Immunoglobulin G (IgG) is composed of discrete domains with known functions, providing a template for engineering antibody fragments with desired imaging properties. Here, we engineered antibody-based imaging probes, consisting of different combinations of antibody domains, labeled them with the near-infrared fluorescent dye IRDye800CW, and evaluated their in vivo imaging properties. Antibody-based imaging probes were based on an anti-HER3 antigen binding fragment (Fab) isolated using phage display. Methods: We constructed six anti-HER3 antibody-based imaging probes: a single chain variable fragment (scFv), Fab, diabody, scFv-C_H3, scFv-Fc,

SUBMITTER: El-Sayed A 

PROVIDER: S-EPMC6160764 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature