Exploring Microtubule-Dependent Cellulose-Synthase-Complex Movement with High Precision Particle Tracking.

ABSTRACT: Cellulose synthesis at the plasma membrane is a critical process in plant growth and development. The displacement of cellulose synthase complexes (CSCs) by the rigid cellulose polymers they produce is a measure of enzyme activity. Connections between cortical microtubules and CSCs have been identified but it remains unclear how these affect CSC displacement speed. In this study, we applied a high throughput automated particle tracking method using near-total internal reflection fluorescence microscopy to measure the speed of CSCs. We found CSC speeds did not vary according to their proximity to microtubules, and that inhibiting microtubule polymerization could have opposite effects on CSC speed, depending on the nature of inhibition. While CSC speed increased in the temperature-sensitive

SUBMITTER: Woodley M 

PROVIDER: S-EPMC6161165 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature