Cell type-specific expression profiling unravels the development and evolution of stinging cells in sea anemone.

ABSTRACT:

Background

Cnidocytes are specialized cells that define the phylum Cnidaria. They possess an "explosive" organelle called cnidocyst that is important for prey capture and anti-predator defense. An extraordinary morphological and functional complexity of the cnidocysts has inspired numerous studies to investigate their structure and development. However, the transcriptomes of the cells bearing these unique organelles are yet to be characterized, impeding our understanding of the genetic basis of their biogenesis.

Results

In this study, we generated a nematocyte reporter transgenic line of the sea anemone Nematostella vectensis using the CRISPR/Cas9 system. By using a fluorescence-activated cell sorter (FACS), we have characterized cell type-specific transcriptomic profiles of

SUBMITTER: Sunagar K 

PROVIDER: S-EPMC6161364 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature