Marsdenia tenacissima extract induces apoptosis and suppresses autophagy through ERK activation in lung cancer cells.

ABSTRACT:

Background

Marsdenia tenacissima is an herb medicine which has been utilized to treat malignant diseases for decades. The M. tenacissima extract (MTE) shows significant anti-proliferation activity against non-small cell lung cancer (NSCLC) cells, but the underlying mechanisms remain unclear. In this study, we explored the potential anti-proliferation mechanisms of MTE in NSCLC cells in relation to apoptosis as well as autophagy, which are two critical forms to control cancer cell survival and death.

Methods

The proliferation of H1975 and A549 cells was evaluated by MTT assay. Cell apoptosis was assessed by Annexin V and PI staining, Caspase 3 expression and activity. Autophagy flux proteins were detected by Western blot with or without autophagy inducer and inh

SUBMITTER: Jiao YN 

PROVIDER: S-EPMC6161462 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature