Role of putative voltage-sensor countercharge D4 in regulating gating properties of Ca_V1.2 and Ca_V1.3 calcium channels.

ABSTRACT: Voltage-dependent calcium channels (Ca_V) activate over a wide range of membrane potentials, and the voltage-dependence of activation of specific channel isoforms is exquisitely tuned to their diverse functions in excitable cells. Alternative splicing further adds to the stunning diversity of gating properties. For example, developmentally regulated insertion of an alternatively spliced exon 29 in the fourth voltage-sensing domain (VSD IV) of Ca_V1.1 right-shifts voltage-dependence of activation by 30 mV and decreases the current amplitude several-fold. Previously we demonstrated that this regulation of gating properties depends on interactions between positive gating charges (R1, R2) and a negative countercharge (D4) in VSD IV of Ca_V1.1. Here we investigated

SUBMITTER: Coste de Bagneaux P 

PROVIDER: S-EPMC6161609 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature