Separation of phenolic acids by centrifugal partition chromatography.

ABSTRACT: Phenolic acids are ubiquitous biomolecules exhibiting a wide range of physiological properties, with application in the pharmaceutical and nutraceutical fields. In this work, aqueous biphasic systems (ABS) formed by polyethylene glycol and sodium polyacrylate, and inorganic salts or ionic liquids as electrolytes, were applied to the purification of caffeic, ferulic and protocatechuic acids, followed by the use of centrifugal partition chromatography (CPC) to reinforce the fractionation process scale-up. In single-step experiments in ABS, high selectivities (S_FA/CA = 12.09; S_CA/PA = 6.32; S_FA/PA = 1.91) and adequate partition coefficients (K_CA = 2.78 ± 0.20; K_PA = 0.44 ± 0.04; K_FA = 0.23 ± 0.01) were achieved using ABS formed

SUBMITTER: Santos JHPM 

PROVIDER: S-EPMC6161811 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature