An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems.
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ABSTRACT:
SUBMITTER: Ferreira AM
PROVIDER: S-EPMC6161812 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
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An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems.
Ferreira Ana M AM Passos Helena H Okafuji Akiyoshi A Tavares Ana P M APM Ohno Hiroyuki H Freire Mara G MG Coutinho João A P JAP
Green chemistry : an international journal and green chemistry resource : GC 20180215 6
Thermoreversible aqueous biphasic systems (ABS) composed of ammonium-based zwitterions (ZIs) and polymers are here disclosed to act as integrated bioreaction-separation processes. The biocatalytic reaction involving laccase occurs in homogeneous media, after which small changes in temperature induce the formation of two phases and the complete separation of the enzyme from the products in a single-step. These systems also allow the recover and reuse of the enzyme, along with the ZI-rich phase, c ...[more]
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