Comparison of trout hepatocytes and liver S9 fractions as in vitro models for predicting hepatic clearance in fish.

ABSTRACT: Isolated hepatocytes and liver S9 fractions have been used to collect in vitro biotransformation data for fish as a means of improving modeled estimates of chemical bioaccumulation. To date, however, there have been few direct comparisons of these 2 methods. In the present study, cryopreserved trout hepatocytes were used to measure in vitro intrinsic clearance rates for 6 polycyclic aromatic hydrocarbons (PAHs). These rates were extrapolated to estimates of in vivo intrinsic clearance and used as inputs to a well stirred liver model to predict hepatic clearance. Predicted rates of hepatic clearance were then evaluated by comparison with measured rates determined previously using isolated perfused livers. Hepatic clearance rates predicted using hepatocytes were in good agreement with measur

SUBMITTER: Fay KA 

PROVIDER: S-EPMC6162060 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature